pCS3 (ATCC® 39142)

Applications: vector for other uses  /  Depositors: Cetus Corp., WJ Scanlon, Cetus Corp.

Permits and Restrictions

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Designations pCS3
Depositors Cetus Corp., WJ Scanlon, Cetus Corp.
Biosafety Level 1
Host
Distribution host: Escherichia coli MM294
Vector Information
Size (kb): 0E-16
Vector: pCS3 (plasmid)
Construction: pEW27, pOP9
Marker(s):ampR,tetR
Construct size (kb): 0.0
Features: marker(s): ampR, tetR
replicon: ColE1
Applications
vector for other uses
Comments
This should NOT be grown over 30C; it is best between 28-30C.
Gives high copy number at high temperature.
This contains the replication origin for high copy number at high temperature of pEW27 (ATCC 37124). pOP9 was constructed by cloning the EcoRI/PvuII fragment containing the origin of pOP6 into pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28.0°C
References

Wong EM, et al. Temperature-sensitive copy number mutants of ColE1 are located in an untranslated region of the plasmid genome. Proc. Natl. Acad. Sci. USA 79: 3570-3574, 1982. PubMed: 6179081

Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet