YIp358 (ATCC® 37751)

Applications: YI-type (integrating) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

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Designations YIp358
Depositors CJ Lusty
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0999999046325680
Vector: YIp358 (plasmid)
Promoters: Promoter none
Construction: YEp358 (ATCC 37733), YIp352
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1
YI-type (integrating) shuttle vector
promoter-cloning vector
shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SacI-1; KpnI-1; SmaI-3; BamHI-3; XbaI-3; SalI-3; PstI-1; SphI-1; HindIII-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37749 - 37751) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'G AAT TCG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
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