YIp358 (ATCC® 37751)

Applications: YI-type (integrating) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

View Permits

Designations YIp358
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 7.0999999046325680
Vector: YIp358 (plasmid)
Promoters: Promoter none
Construction: YEp358 (ATCC 37733), YIp352
Marker(s):URA3,ampR
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SacI-1; KpnI-1; SmaI-3; BamHI-3; XbaI-3; SalI-3; PstI-1; SphI-1; HindIII-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37749 - 37751) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'G AAT TCG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet