pG195/T1 (ATCC® 37671)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: A Tzagoloff

Permits and Restrictions

View Permits

Designations pG195/T1
GenBank Number

J02763

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Vector
Construct size (kb): 18.5
Insert
DNA: genomic
Insert lengths(kb): 7.800000190734863
Gene product: ribosomal protein, mitochondrial, 37 kDa [MRP1]
Insert Size (kb) 7.800
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
The insert contains the following sites separated by (approximate kb): Sau3A1- 0.14- XhoI- 0.27- BstI- 0.33- SacI- 0.26- Sau3A1- 0.54- BamHI- 0.6- HindIII.
Restriction digests of the clone give the following sizes (kb): XbaI/AvaI--9.4, 6.8, 2.5; KpnI--10, 6, 2.4; AvaI--18.5; EcoRI/SalI--4.2, 4, 3.7, 3.25, 2.2, 0.98.
The insert includes a continuous open reading frame of 963 nt encoding a protein of 37 kDa. Most of this is included in a 1.0 kb Sau3A1 fragment which is sufficient to complement deletion mutants.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Myers AM, et al. Assembly of the mitochondrial membrane system. MRP1 and MRP2, two yeast nuclear genes coding for mitochondrial ribosomal proteins. J. Biol. Chem. 262: 3388-3397, 1987. PubMed: 3029111

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