pLKC482 (ATCC® 37596)

Applications: contains easily purifiable cassette(s) for constructionvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: AA Tiedman, A Shiau

Permits and Restrictions

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Designations pLKC482
Depositors AA Tiedman, A Shiau
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.8999996185302730
Vector: pLKC482 (plasmid)
Promoters: Promoter none
Construction: A lacZY plasmid, Tn5
Construct size (kb): 9.899999618530273
Features: insert detection: lacZ
marker(s): ampR, kanR
promoter: none
replicon: pMB1
enhancer: none
contains easily purifiable cassette(s) for construction
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
The 6.3 kb lacZY-kanR cassette is released with SmaI.
Restriction digests of the clone give the following sizes (kb): EcoRI--10.0; BamHI--10.0; PstI--5.5, 4.4; HindIII--10.2; SmaI--6.3, 3.6.
One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the Escherichia coli genome.
A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Tiedeman AA, Smith JM. lacZY gene fusion cassettes with kanR resistance. Nucleic Acids Res. 16: 3587, 1988. PubMed: 3131742

Amelia A Tiedman, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet