pC22 (ATCC® 37493)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: M Van Montagu

Permits and Restrictions

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Designations pC22
Depositors M Van Montagu
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 17.5000000000000000
Vector: pC22 (cosmid)
Promoters: Promoter nos
Construction: pBR322, pRiHR1, R702, pTiB6S3
Marker(s):spcR,strR,CbR
Construct size (kb): 17.5
Features: marker(s): CbR, spcR, strR
promoter: nos
replicon: pMB1, pRiHR1
Applications
shuttle vector
vector for constructing genomic libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A binary cosmid vector designed to introduce genomic libraries into plant cells.
Media ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
References

Simoens C, et al. A binary vector for transferring genomic libraries to plants. Nucleic Acids Res. 14: 8073-8089, 1986. PubMed: 3534794

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the cosmid.