pC22 (ATCC® 37493)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: M Van Montagu

Permits and Restrictions

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Designations pC22
Depositors M Van Montagu
Biosafety Level 1
Vector Information
Size (kb): 17.5000000000000000
Vector: pC22 (cosmid)
Promoters: Promoter nos
Construction: pBR322, pRiHR1, R702, pTiB6S3
Marker(s):spcR,strR,CbR
Construct size (kb): 17.5
Features: marker(s): CbR, spcR, strR
promoter: nos
replicon: pMB1, pRiHR1
Applications
shuttle vector
vector for constructing genomic libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A binary cosmid vector designed to introduce genomic libraries into plant cells.
Media Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
References

Simoens C, et al. A binary vector for transferring genomic libraries to plants. Nucleic Acids Res. 14: 8073-8089, 1986. PubMed: 3534794

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the cosmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37493