pLB1 (ATCC® 37393)

Organism: Azotobacter vinelandii Lipman  /  Clone Type: Clone  /  Depositors: PE Bishop

Permits and Restrictions

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Designations pLB1
Species Azotobacter vinelandii Lipman
Depositors PE Bishop
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 2.599999904632568
Gene product: dinitrogenase reductase [nifK]
Insert Size (kb) 2.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Insert contains PstI, HindIII, AvaI and SalI sites.
The insert contains the following sites separated by (bp)(approx): EcoRI- 175- PstI, HindIII- 520- PstI- 175- AvaI- 300- PstI- 175- SalI- 175- SalI- 910- SalI- 130- EcoRI.
This is a clone of the nifK gene, including the wild type alleles for nif-6 and nif-38.
References

Bishop PE, et al. Molecular cloning of nif DNA from Azotobacter vinelandii. J. Bacteriol. 162: 21-28, 1985. PubMed: 3884589