pLB1 (ATCC® 37393)

Organism: Azotobacter vinelandii Lipman  /  Clone Type: Clone  /  Depositors: PE Bishop

Permits and Restrictions

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Designations pLB1
Species Azotobacter vinelandii Lipman
Depositors PE Bishop
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 2.599999904632568
Gene product: dinitrogenase reductase [nifK]
Insert Size (kb) 2.600
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Insert contains PstI, HindIII, AvaI and SalI sites.
The insert contains the following sites separated by (bp)(approx): EcoRI- 175- PstI, HindIII- 520- PstI- 175- AvaI- 300- PstI- 175- SalI- 175- SalI- 910- SalI- 130- EcoRI.
This is a clone of the nifK gene, including the wild type alleles for nif-6 and nif-38.
References

Bishop PE, et al. Molecular cloning of nif DNA from Azotobacter vinelandii. J. Bacteriol. 162: 21-28, 1985. PubMed: 3884589

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