pE194 (ATCC® 37128)

Other IDs:

Nucleotide (GenBank) : VB0131 pE194 cloning vector.

 /  Applications: shuttle vector  /  Depositors: DM Ellis

Permits and Restrictions

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Designations pE194
Other IDs

Nucleotide (GenBank) : VB0131 pE194 cloning vector.

Depositors DM Ellis
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.7
Vector: pE194 (plasmid)
Promoters: Promoter none
Construction: natural isolate
Marker(s):eryR
Construct size (kb): 3.7
Features: marker(s): eryR
promoter: none
replicon: pE194
terminator: none
enhancer: none
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): AccI--3.8; PstI--3.8.
Temperature sensitive.
For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin.
natural isolate from Staphylococcus aureus
confers resistance to macrolide, lincosamide, and streptogramin type B "MLS" antibiotics.
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 30.0°C
References

Horinouchi S, Weisblum B. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. USA 77: 7079-7083, 1980. PubMed: 6938954

Iordanescu S. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75, 1977. PubMed: 830649

Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975

Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814, 1982. PubMed: 6279574

Shipped frozen