Nucleotide (GenBank) :
pE194 cloning vector.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 3.7
Vector: pE194 (plasmid)
Promoters: Promoter none
Construction: natural isolate
Construct size (kb): 3.7
Features: marker(s): eryR
Restriction digests of the clone give the following sizes (kb): AccI--3.8; PstI--3.8.
For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin.
natural isolate from Staphylococcus aureus
confers resistance to macrolide, lincosamide, and streptogramin type B "MLS" antibiotics.
Horinouchi S, Weisblum B. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. USA 77: 7079-7083, 1980. PubMed: 6938954
Iordanescu S. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75, 1977. PubMed: 830649
Weisblum B, et al. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643, 1979. PubMed: 104975
Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814, 1982. PubMed: 6279574