1D11.16.8 (ATCC® HB-9849)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1
Applications
The antibody reacts with both TGF-beta1 and TGF-beta2, and can inhibit their biological activities.
Storage Conditions liquid nitrogen vapor phase
Derivation Animals were immunized with a bovine TGF-beta2. Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against transforming growth factor beta2 (TGF-beta2)
Cellular Products
immunoglobulin; monoclonal antibody; against transforming growth factor beta2 (TGF-beta2)
Tumorigenic Yes
Effects Yes, forms ascites in pristane primed BALB/c mice
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • O.1 mM Non-Essential Amino Acids (NEAA)
  • fetal bovine serum to a final concentration of 10%

Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C
Atmosphere: 5% CO2

Isotype IgG1, IgG1
Name of Depositor Celtrix Pharmaceuticals, Inc.
References

Dasch JR, et al. Monoclonal antibodies which bind both transforming growth factors .beta.1 and .beta.2 and methods of use. US Patent 5,571,714 dated Nov 5 1996

Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,772,998 dated Jun 30 1998

Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Basic Documentation
References

Dasch JR, et al. Monoclonal antibodies which bind both transforming growth factors .beta.1 and .beta.2 and methods of use. US Patent 5,571,714 dated Nov 5 1996

Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,772,998 dated Jun 30 1998

Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm