BB7.1 (ATCC® HB-56)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody appears to react with an epitope coded by the HLA-B*0702 allele.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with papain solubilized HLA B7 antigen.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B7
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B7
Comments
The antibody appears to react with an epitope coded by the HLA-B*0702 allele.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 1 to 2 days
Isotype IgG1
Name of Depositor P Parham
References

Trapani JA, et al. Immunoradiometric assay for the rapid detection of HLA-B27. Immunol. Cell Biol. 66: 215-219, 1988. PubMed: 3155158

Toubert A, et al. Epitope mapping of HLA-B27 and HLA-B7 antigens by using intradomain recombinants. J. Immunol. 141: 2503-2509, 1988. PubMed: 2459214

McCutcheon JA, Lutz CT. Mutagenesis around residue 176 on HLA-B*0702 characterizes multiple distinct epitopes for anti-HLA antibodies. Hum. Immunol. 35: 125-131, 1992. PubMed: 1283748

McCutcheon JA, et al. HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues. Hum. Immunol. 36: 69-75, 1993. PubMed: 7681814

Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Trapani JA, et al. Immunoradiometric assay for the rapid detection of HLA-B27. Immunol. Cell Biol. 66: 215-219, 1988. PubMed: 3155158

Toubert A, et al. Epitope mapping of HLA-B27 and HLA-B7 antigens by using intradomain recombinants. J. Immunol. 141: 2503-2509, 1988. PubMed: 2459214

McCutcheon JA, Lutz CT. Mutagenesis around residue 176 on HLA-B*0702 characterizes multiple distinct epitopes for anti-HLA antibodies. Hum. Immunol. 35: 125-131, 1992. PubMed: 1283748

McCutcheon JA, et al. HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues. Hum. Immunol. 36: 69-75, 1993. PubMed: 7681814

Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015