BB7.1 (ATCC® HB-56)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody appears to react with an epitope coded by the HLA-B*0702 allele.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with papain solubilized HLA B7 antigen.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B7
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B7
Comments
The antibody appears to react with an epitope coded by the HLA-B*0702 allele.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 1 to 2 days
Isotype IgG1
Name of Depositor P Parham
References

Trapani JA, et al. Immunoradiometric assay for the rapid detection of HLA-B27. Immunol. Cell Biol. 66: 215-219, 1988. PubMed: 3155158

Toubert A, et al. Epitope mapping of HLA-B27 and HLA-B7 antigens by using intradomain recombinants. J. Immunol. 141: 2503-2509, 1988. PubMed: 2459214

McCutcheon JA, Lutz CT. Mutagenesis around residue 176 on HLA-B*0702 characterizes multiple distinct epitopes for anti-HLA antibodies. Hum. Immunol. 35: 125-131, 1992. PubMed: 1283748

McCutcheon JA, et al. HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues. Hum. Immunol. 36: 69-75, 1993. PubMed: 7681814

Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Trapani JA, et al. Immunoradiometric assay for the rapid detection of HLA-B27. Immunol. Cell Biol. 66: 215-219, 1988. PubMed: 3155158

Toubert A, et al. Epitope mapping of HLA-B27 and HLA-B7 antigens by using intradomain recombinants. J. Immunol. 141: 2503-2509, 1988. PubMed: 2459214

McCutcheon JA, Lutz CT. Mutagenesis around residue 176 on HLA-B*0702 characterizes multiple distinct epitopes for anti-HLA antibodies. Hum. Immunol. 35: 125-131, 1992. PubMed: 1283748

McCutcheon JA, et al. HLA-B*0702 antibody epitopes are affected indirectly by distant antigen residues. Hum. Immunol. 36: 69-75, 1993. PubMed: 7681814

Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015