MECA-89 (ATCC® HB-292)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1)
Cellular Products
immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1)
Comments
Animals were immunized with endothelial cells from mouse mesenteric and peripheral lymph nodes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer).
Unlike MECA-367, MECA-89 does not inhibit alpha 4, beta 7 dependent binding in vitro.
Complete Growth Medium
Culture Medium: RPMI 1640 with 4.5 g/L glucose, 15 mM HEPES, 2 mM L-glutamine,
0.01 mM non-essential amino acids, and 0.05 mM 2-mercaptoethanol, 90%; fetal
bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor EC Butcher, DP Andrew
References

Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297