Animals were immunized with human lung fibroblasts.
The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas.
The F19 antigen is not detected on SV40 transformed fibroblasts.
During scar formation, the antigen is expressed in the scar but not in the surrounding tissue.
The F19 antigen is also often expressed in the normal stroma surrounding the growth of tumors.
A culture deposited with the ATCC as HB-8269 in March of 1983 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as ATCC CRL-2733. The original patent deposit is available as HB-8269.