Cryptococcus neoformans (Sanfelice) Vuillemin (ATCC® CNKO-PS)

Alternate State: Filobasidiella neoformans Kwon-Chung  /  Strain Designations: CnKOv1.0  /  Product Format: frozen

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Deposited As Cryptococcus neoformans (Sanfelice) Vuillemin, anamorph
Strain Designations CnKOv1.0
Alternate State Filobasidiella neoformans Kwon-Chung
Application
Emerging infectious disease research
Biosafety Level 2
Product Format frozen
Type Strain no
Genotype MATalpha cnx####::natR
Preceptrol® no
Comments
Cryptococcus neoformans Genome ORF Knockout Collection Version 1.0 (CnKOv1,0) The Madhani laboratory at the University of California-San Francisco recently generated deletions in approximately 1,200 Open Reading Frames (ORFs) (i.e., about 20% of the genome ORFs) of strain H99 (serotype A, var. grubii) of this organism. Each ORF deletion is made through homologous recombination in which a genome ORF is replaced by a PCR-generated DNA fragment containing selection marker natR and a unique barcode of 40 base pairs. The genotype of each of these gene knockout strains is MATalpha cnx####::natR, wherein x refers to an alphabet and #### to a 4-digit number and natR represents the gene that confers resistance to nourseothricin. For further information on the methodologies in making gene knockouts via homologous recombination and generation of 40-bp unique barcodes, see the publications: Davidson et al., Fungal Genetics & Biology 29: 38-48 (2000); and Nelson et al., Genetics 157: 935-947 (2001). Strains of Cryptococcus neoformans Genome ORF Knockout Collection (CnKOv1.0) are distributed as a set of fourteen 96-well standard microtiter plates (called CNKO-PS) or single strains can be ordered individually. Please contact us at (800) 638-6597 or tech@atcc.org if you have any questions.
Medium Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Name of Depositor H Madhani
Special Collection NSF - Mycology
Chain of Custody
H Madhani
References

Davidson RC, et al. Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. Fungal Genet. Biol. 29: 38-48, 2000. PubMed: 10779398

Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis. Genetics 157: 935, 2001.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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