Cryptococcus neoformans (Sanfelice) Vuillemin (ATCC® CNKO-PS)

Alternate State: Filobasidiella neoformans Kwon-Chung  /  Strain Designations: CnKOv1.0  /  Product Format: frozen

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Strain Designations CnKOv1.0
Alternate State Filobasidiella neoformans Kwon-Chung
Application
Emerging infectious disease research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype MATalpha cnx####::natR
Preceptrol® no
Comments
Cryptococcus neoformans Genome ORF Knockout Collection Version 1.0 (CnKOv1,0).  The Madhani laboratory at the University of California-San Francisco recently generated deletions in approximately 1,200 Open Reading Frames (ORFs) (i.e., about 20% of the genome ORFs) of strain H99 (serotype A, var. grubii) of this organism. Each ORF deletion is made through homologous recombination in which a genome ORF is replaced by a PCR-generated DNA fragment containing selection marker natR and a unique barcode of 40 base pairs. The genotype of each of these gene knockout strains is MATalpha cnx####::natR, wherein x refers to an alphabet and #### to a 4-digit number and natR represents the gene that confers resistance to nourseothricin. For further information on the methodologies in making gene knockouts via homologous recombination and generation of 40-bp unique barcodes, see the publications: Davidson et al., Fungal Genetics & Biology 29: 38-48 (2000); and Nelson et al., Genetics 157: 935-947 (2001). Strains of Cryptococcus neoformans Genome ORF Knockout Collection (CnKOv1.0) are distributed as a set of fourteen 96-well standard microtiter plates (called CNKO-PS) or single strains can be ordered individually. Please contact us at (800) 638-6597 or tech@atcc.org if you have any questions.
Medium ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25°C
Name of Depositor H Madhani
Special Collection NSF - Mycology
Chain of Custody
H Madhani
References

Davidson RC, et al. Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. Fungal Genet. Biol. 29: 38-48, 2000. PubMed: 10779398

Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis. Genetics 157: 935, 2001.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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