Cryptococcus neoformans (Sanfelice) Vuillemin (ATCC® 32045)

Alternate State: Filobasidiella neoformans Kwon-Chung  /  Strain Designations: CBS 132 [CCRC 20528, DBVPG 6010, IFO 0608, NRRL Y-2534]  /  Product Format: freeze-dried

Strain Designations CBS 132 [CCRC 20528, DBVPG 6010, IFO 0608, NRRL Y-2534]
Alternate State Filobasidiella neoformans Kwon-Chung
Application
control strain for identification
Biomedical Research and Development Material
Emerging infectious disease research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® yes
Morphology After days on Emmons’ modification of Sabouraud’s medium at 25°C, colony is cream, butyrous, smooth and mucoid. Cells are globose, subglobose or irregular, single or in pairs. Pseudohyphae are not observed.
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTAC


D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC

Morphology After days on Emmons’ modification of Sabouraud’s medium at 25°C, colony is cream, butyrous, smooth and mucoid. Cells are globose, subglobose or irregular, single or in pairs. Pseudohyphae are not observed.
Name of Depositor CBS
Chain of Custody
ATCC <-- CBS <-- A Giordana
Isolation
Fermenting fruit juice
Cross References

Nucleotide (GenBank) : EU240005 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729166 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : AF075484 26S ribosomal RNA gene, partial sequence

Nucleotide (GenBank) : AF218975 Filobasidiella neoformans var. neoformans strain ATCC 32045T 5.8S

References

Vanbreuseghem R, et al. Experiences with BAY b 5097. Mykosen 18: 199-211, 1975. PubMed: 1099443

Sanfelice F. Sull'azione patogena dei blastomiceti. Ann. Ig. Sper. 5: 239-262, 1895.

Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082

Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334

Diaz MR, et al. Comparative analysis of the intergenic spacer regions and population structure of the species complex of the pathogenic yeast Cryptococcus neoformans. FEMS Yeast Res 5: 1129-1140, 2005. PubMed: 16061425

Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266

Chen YC, et al. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J. Clin. Microbiol. 39: 4042-4051, 2001. PubMed: 11682528

Biswas SK, et al. Molecular phylogenetics of the genus Rhodotorula and related basidiomycetous yeasts inferred from the mitochondrial cytochrome b gene. Int. J. Syst. Evol. Microbiol. 51: 1191-1199, 2001. PubMed: 11411687

Sugita T, Ikeda R, Shinoda T. Diversity among strains of Cryptococcus neoformans var. gattii as revealed by a sequence analysis of multiple genes and a chemotype analysis of capsular polysaccharide. Microbiol Immunol 45: 757-768, 2001. PubMed: 11791669

Butler MI, Goodwin TJD, Poulter RTM. A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans. Yeast 18: 1365-1370, 2001. PubMed: 11746598

Chen YC, et al. Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA genes. J. Clin. Microbiol. 38: 2302-2310, 2000. PubMed: 10834993

Cogliati M, et al. Origin of Cryptococcus neoformans var. neoformans diploid strains. J Clin Microbiol 39: 3889-3894, 2001. PubMed: 11682503