c9581 (ATCC® 71229)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: R Hyman

Designations c9581
GenBank Number

U18778

U18796

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R Hyman
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: GPA2
Gene name: nucleotide binding regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: SRB4
Gene name: RNA polymerase II suppressor
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: PRO3
Gene name: delta 1-pyrroline-5-carboxylate reductase
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GCD11
Gene name: gamma subunit of translational initiation factor eIF-2
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: CHO1
Gene name: phosphatidylserine synthase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 23 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: GAL83
Gene name: glucose repression protein
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 11 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: YPT8
Gene name: Rab-family GTPase activating protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GLN3
Gene name: positive nitrogen regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: DNA Seq. Acc.: U18778
Nucleotides ? of the insert correspond to
nucleotides 37895-66030 of U18778.
DNA Seq. Acc.: U18796
Nucleotides ? of the insert correspond to
nucleotides 1-14353 of U18796.
Gene product: phosphatidylserine synthase [CHO1]
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet