c9581 (ATCC® 71229)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: R Hyman

Permits and Restrictions

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Designations c9581
GenBank Number

U18778

U18796

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R Hyman
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: GPA2
Gene name: nucleotide binding regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: SRB4
Gene name: RNA polymerase II suppressor
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: PRO3
Gene name: delta 1-pyrroline-5-carboxylate reductase
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GCD11
Gene name: gamma subunit of translational initiation factor eIF-2
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: CHO1
Gene name: phosphatidylserine synthase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 23 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: GAL83
Gene name: glucose repression protein
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 11 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: YPT8
Gene name: Rab-family GTPase activating protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GLN3
Gene name: positive nitrogen regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: DNA Seq. Acc.: U18778
Nucleotides ? of the insert correspond to
nucleotides 37895-66030 of U18778.
DNA Seq. Acc.: U18796
Nucleotides ? of the insert correspond to
nucleotides 1-14353 of U18796.
Gene product: phosphatidylserine synthase [CHO1]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet