c8543 (ATCC® 71210)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: L Riles, MV Olson

Permits and Restrictions

View Permits

Designations c8543
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors L Riles, MV Olson
Vector
Construct size (kb): 45.29999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: CDC3
Contains complete coding sequence?: Y
Chromosome: XII; Localization: XII R, 225 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: REC102
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: MID2
Gene name: mating process protein
Contains complete coding sequence?: Y
Chromosome: XII
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 37.7
Cross references:
Insert lengths(kb): 37.70000076293945
Gene product: mating process protein [CDC3]
Insert Size (kb) 37.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Product Sheet
Product Sheet
Product Sheet