Saccharomyces cerevisiae Hansen (ATCC® 96408)

Strain Designations: AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]  /  Product Format: frozen

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Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Strain Designations AB1380 carrying a 540-kb YAC with genes for human [D142H8, yGART2]
Biosafety Level 1
Product Format frozen
Type Strain no
Preceptrol® no
Comments
Consists of 5 SfiI fragments, 3 internal ones (70, 110, and 300 kb) and the YAC ends (50 and 70 kb).
Parental, unfragmented YAC

 

The smallest fragment found to contain all the exons for GART is a 45 kb BssHII fragment.

The GART gene is toward the acentric end of the clone and the IFNGT1 gene is within 150 kb of the centric end.

 

When transferred into CHO cells, the clone complements the purine requirement of CHO Ade-C cells (lacking PRGS/GARS) and Ade-CG cells (lacking PRGS/GARS and PAIS/AIRS).

Both ends of the insert map to chromosome 21, suggesting a non-chimeric insert in this YAC.

DNA: genomic
Insert lengths (kb): 540.0
Tissue: lymphoblastoid CGM1 cell line
Gene Product: interferon, gamma transducer 1 (interferon, gamma receptor accessory factor) [GART]

 

Medium ATCC® Medium 1798: aAHC medium for YAC clones
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor S Pestka
Special Collection NCRR Contract
References

Gnirke A, et al. Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes. EMBO J. 10: 1629-1634, 1991. PubMed: 2050105

Gnirke A, Huxley C. Transfer of the human HPRT and GART genes from yeast to mammalian cells by microinjection of YAC DNA. Somatic Cell Mol. Genet. 17: 573-580, 1991. PubMed: 1767336

Cook JR, et al. Yeast artificial chromosome fragmentation vectors that utilize URA3 selection. Genet. Anal. Tech. Appl. 10: 109-112, 1993. PubMed: 8204303

Cook JR, et al. Sublocalization of the human interferon-gamma receptor accessory factor gene and characterization of accessory factor activity by yeast artificial chromosome fragmentation. J. Biol. Chem. 269: 7013-7018, 1994. PubMed: 8120065

Soh J, et al. Identification of a yeast artificial chromosome clone encoding an accessory factor for the human interferon gamma receptor: evidence for multiple accessory factors. Proc. Natl. Acad. Sci. USA 90: 8737-8741, 1993. PubMed: 8378357

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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