Saccharomyces cerevisiae Hansen, teleomorph (ATCC® 20796)

Strain Designations: pYLUIGF2-14 [AB110]  /  Product Format: frozen

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Strain Designations pYLUIGF2-14 [AB110]
Application
Produces superoxide dismutase and other polypeptides
Biosafety Level 1
Product Format frozen
Type Strain no
Preceptrol® no
Comments
Produces superoxide dismutase and other polypeptides
The insert contains human superoxide dismutase fused to the amino-terminus of insulin-like growth factor 2 with a methionine codon at the junction between the two sequences.

 

A 0.51 kb EcoRI/BamHI fragment containing the IGF2 and alpha-factor terminator was ligated with the 1.83 kb BamHI/EcoRI fragment containing the ADH2-GAP promoter and SOD. The resulting 2.34 kb BamHI fragment was cloned into pAB24.

DNA: cDNA
Gene Product: insulin-like growth factor 2 [IGF2]
Alleles: A1, A2, B1, B2
Medium ATCC® Medium 1212: Yeast synthetic minimal medium
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Chiron Corp.
References

Cousens LS, et al. Expression using fused genes providing for protein product. US Patent 4,751,180 dated Jun 14 1988

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation