Produces superoxide dismutase and other polypeptides
The insert contains human superoxide dismutase fused to the amino-terminus of human proinsulin with a methionine codon at the junction between the two sequences.
A 1.3 kb fragment containing the ADH2-GAP promoter was ligated to the 1.7 kb fragment containing SOD1-proinsulin fusion sequences and GAP terminator. This 3 kb cassette was then cloned into pC1/1.
The superoxide dismutase sequences were isolated from a human liver cDNA library. The proinsulin sequences were chemically synthesized using yeast-preferred codons.
Gene Product: insulin [INS]
Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, B1, A1, A2, A2, A2, B2