Saccharomyces cerevisiae Hansen (ATCC® 20745)

Strain Designations: pYAS11 [2150-2-3]  /  Product Format: frozen

Strain Designations pYAS11 [2150-2-3]
Produces superoxide dismutase and other polypeptides
Biosafety Level 1
Product Format frozen
Type Strain no
Preceptrol® no
Produces superoxide dismutase and other polypeptides
The insert contains human superoxide dismutase fused to the amino-terminus of human proinsulin with a methionine codon at the junction between the two sequences.


A 1.3 kb fragment containing the ADH2-GAP promoter was ligated to the 1.7 kb fragment containing SOD1-proinsulin fusion sequences and GAP terminator. This 3 kb cassette was then cloned into pC1/1.

The superoxide dismutase sequences were isolated from a human liver cDNA library. The proinsulin sequences were chemically synthesized using yeast-preferred codons.


Gene Product: insulin [INS]
Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, B1, A1, A2, A2, A2, B2


Medium ATCC® Medium 1212: Yeast synthetic minimal medium
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Chiron Corp.

Cousens LS, et al. Expression using fused genes providing for protein product. US Patent 4,751,180 dated Jun 14 1988