Saccharomyces cerevisiae Hansen, teleomorph (ATCC® 20745™)

Strain Designations: pYAS11 [2150-2-3]  /  Product Format: frozen

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Strain Designations pYAS11 [2150-2-3]
Application
Produces superoxide dismutase and other polypeptides
Biosafety Level 1
Product Format frozen
Type Strain no
Comments
Produces superoxide dismutase and other polypeptides
The insert contains human superoxide dismutase fused to the amino-terminus of human proinsulin with a methionine codon at the junction between the two sequences.

 

A 1.3 kb fragment containing the ADH2-GAP promoter was ligated to the 1.7 kb fragment containing SOD1-proinsulin fusion sequences and GAP terminator. This 3 kb cassette was then cloned into pC1/1.

The superoxide dismutase sequences were isolated from a human liver cDNA library. The proinsulin sequences were chemically synthesized using yeast-preferred codons.

 

DNA: cDNA
Gene Product: insulin [INS]
Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, B1, A1, A2, A2, A2, B2

 
Medium ATCC® Medium 1212: Yeast synthetic minimal medium
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Chiron Corp.
References

Cousens LS, et al. Expression using fused genes providing for protein product. US Patent 4,751,180 dated Jun 14 1988

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS