Saccharomyces cerevisiae Hansen (ATCC® 20663)

Strain Designations: pCGS261 [CGY528]  /  Product Format: frozen

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Strain Designations pCGS261 [CGY528]
Application
Carries a GAL1 promoter of the yeast galactokinase gene for use in expressing a desired protein, in this case human interferon
Biosafety Level 1
Product Format frozen
Type Strain no
Genotype MATalpha ura3-52 leu2-3 leu2-112 trpl-289 his3-delta1 met2 cyh(r) [cir0] Carring a GAL1 promoter of the yeast galactokinase gene for expressing human interferon
Preceptrol® no
Comments
Carries a GAL1 promoter of the yeast galactokinase gene for use in expressing a desired protein, in this case human interferon
The insert contains the complete coding sequence plus 5' and 3' untranslated regions.

pGCS261 was constructed from YIp5, a 1.56 kb HpaI/HindIII fragment of the B form of yeast 2 micron plasmid, the GAL1 promoter from Sc4812, and a 1.1 kb HindIII fragment containing the leukocyte interferon sequences.

DNA: cDNA
Insert Lengths (kb): 1.100000023841858
Tissue: lymphocyte, Sendai virus-induced
Gene Product: interferon, type 1, cluster( interferon alpha gene family (leukocyte), interferon, alpha (leukocyte), interferon, type 1, cluster) [IFNA]
Medium ATCC® Medium 2318: 1212 plus 20 mg/L uracil, leucine, tryptophan, histidine and lysine
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Collaborative Research, Inc., DT Moir, Collaborative Research, Inc.
Special Collection ATCC
References

Botstein D, et al. GAL1 yeast promoter linked to non galactokinase gene. US Patent 4,661,454 dated Apr 20 1987

plus histidine (20 mg/L)

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation