MATalpha leu2-3 leu2-112 ura3-52 his4-580 pep4-3
Produces epidermal growth factor
Produces functional product (assayed by receptor binding) of 6 kDa, indicating proper excision of the alpha factor leader sequence.
The 1.75 kb insert consists of the gene for the alpha mating factor in which a central portion has been replaced by the synthetic sequence encoding epidermal growth factor.
The order of the major features in this plasmid is: BamHI - MATalpha/EGF/MATalpha - BamHI - 2 micron ori - LEU2 - 2 micron ori - pMB1 ori - bla.
Insert Lengths (kb): 1.75
Gene Product: epidermal growth factor (urogastrone) [EGF]
Alleles: A1, B1, B2, A2
Target Gene: epidermal growth factor