Saccharomyces cerevisiae Hansen, teleomorph (ATCC® 20658™)

Strain Designations: pYEGF8 [AB103]  /  Product Format: freeze-dried

Strain Designations pYEGF8 [AB103]
Application
produces protein epidermal growth factor urogastrone
Biosafety Level 1
Product Format freeze-dried
Type Strain no
Genotype MATalpha leu2-3 leu2-112 ura3-52 his4-580 pep4-3
Mating Type alpha
Comments
Produces epidermal growth factor
Produces functional product (assayed by receptor binding) of 6 kDa, indicating proper excision of the alpha factor leader sequence.
The 1.75 kb insert consists of the gene for the alpha mating factor in which a central portion has been replaced by the synthetic sequence encoding epidermal growth factor.

The order of the major features in this plasmid is: BamHI - MATalpha/EGF/MATalpha - BamHI - 2 micron ori - LEU2 - 2 micron ori - pMB1 ori - bla.

DNA: Synthetic
Insert Lengths (kb): 1.75
Gene Product: epidermal growth factor (urogastrone) [EGF]
Alleles: A1, B1, B2, A2
Target Gene: epidermal growth factor
Medium ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Name of Depositor Chiron Corp., B Mendez, Chiron Corp.
References

Brake AJ. Secretory expression in eukaryotes. US Patent 4,870,008 dated Sep 26 1989