Trichoderma reesei Simmons, anamorph (ATCC® 26921)

Strain Designations: QM 9414 [3019, CBS 392.92, T.V. B118]  /  Product Format: freeze-dried

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Deposited As Trichoderma viride Persoon : Fries, anamorph
Strain Designations QM 9414 [3019, CBS 392.92, T.V. B118]
Produces 17 beta-hydroxysteroid dehydrogenase Estradiol 17-beta-dehydrogenase-1, Hydroxysteroid dehydrogenase 17 beta, type 1, hydroxysteroid 17-beta dehydrogenase 1
Produces 20 beta-hydroxysteroid dehydrogenase
Produces acetylesterase acetyl esterase
Produces alpha-1,2-glucuronidase
Produces alpha-L-arabinofuranosidase alpha-1,3-arabinosidase
Produces endoglucanase
Produces endoglucanase III EIII
Produces endoglucanase-cellobiohydrolase complex
Produces exo-1,4-beta-D-xylosidase beta-xylosidase
Produces exo-cellobiohydrolase avicelase, endoglucanase I
Produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Produces hydrolase
Produces mannan endo-1,4-beta-mannosidase beta-mannanase, mannanase
Produces paracelsin
Transformation host
Produces extracellular hydrolases
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain


Produces 1.5-2.0 times more cellulase on cellulose medium than ATCC 24449
EIII core protein of endoglucanase
Ethanol- and polyene-sensitive
Mitochondrial genome sequenced strain (University of Sao Paulo, Brazil).
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).
Medium Medium 336: Potato dextrose agar (PDA)
Medium 200: YM agar or YM broth
Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor QM
Chain of Custody
ATCC <-- QM <-- M Mandels 3019
Mutant of ATCC 24449, QM 9123
Cross References

Nucleotide (GenBank) : M81127 Trichoderma reesei TR1 promoter.

Nucleotide (GenBank) : X70232 T. reesei cbh2 gene (promoter region).

Nucleotide (GenBank) : D63514 creA gene for DNA-binding protein, complete coding sequence


Sprey B, Lambert C. Heterogeneity of cellulase complexes from Trichoderma reesei: a preparative isoelectric focusing study of some extracellular hydrolases. FEMS Microbiol. Lett. 23: 227-232, 1984.

Stahlberg J, et al. A binding-site-deficient, catalytically active, core protein of endoglucanase III from the culture filtrate of Trichoderma reesei. Eur. J. Biochem. 173: 179-183, 1988. PubMed: 3356188

Mach RL, et al. Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals. Curr. Genet. 25: 567-570, 1994. PubMed: 8082210

Haab D, et al. Protein hypersecretory Trichoderma reesei mutant RUT C-30 displays increased ethanol and polyene resistance. J. Biotechnol. 29: 97-108, 1993.

Lanisnik T, et al. Fungal 17 beta-hydroxysteroid dehydrogenase. FEMS Microbiol. Lett. 78: 49-52, 1992. PubMed: 1334892

Dominguez JM, et al. Spontaneous aggregation of endoglucanase 1 from Trichoderma reesei QM 9414. Biotechnol. Appl. Biochem. 15: 236-246, 1992.

Takashima S, et al. Cloning of a gene encoding a putative carbon catabolite repressor from Trichoderma reesei. Biosci. Biotechnol. Biochem. 60: 173-176, 1996. PubMed: 8824842

Rogalski J, et al. Affinity chromatography of 1 4-beta glucosidase from Trichoderma reesei QM 9414. Acta Biotechnol. 11: 485-494, 1991.

Messner R, Kubicek CP. Evidence for a single specific beta glucosidase in cell walls from Trichoderma reesei QM9414. Enzyme Microb. Technol. 12: 685-690, 1990.

Estrada P, et al. Kinetic mechanism of beta-glucosidase from Trichoderma reesei QM 9414. Biochim. Biophys. Acta 1033: 298-304, 1990. PubMed: 2107875

Sprey B, Bochem HP. Formation of cross-fractures in cellulose microfibril structure by an endoglucanase-cellobiohydrolase complex from Trichoderma reesei. FEMS Microbiol. Lett. 106: 239-243, 1993. PubMed: 8454189

Bruckner H, Graf H. Paracelsin, a peptide antibiotic containing alpha-aminoisobutyric acid, isolated from Trichoderma reesei Simmons. Part A. Experientia 39: 528-530, 1983. PubMed: 6406260

Przybylski M, et al. Elucidation of structure and microheterogeneity of the polypeptide antibiotics paracelsin and trichotoxin A-50 by fast atom bombardment mass spectrometry in combination with selective in situ hydrolysis. Biomed. Mass Spectrom. 11: 569-582, 1984.

Poutanen K, et al. The hemicellulolytic activities of Trichoderma reesei. Proc. 4th Eur. Congr. Biotechnol. 2: 90, 1987.

Sternberg D. A method for increasing cellulase production by Trichoderma viride. Biotechnol. Bioeng. 18: 1751-1760, 1976.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation