Candida parapolymorpha Suh et Zhou (ATCC® 26012)

Strain Designations: DL-1 [NRRL Y-7560]  /  Product Format: frozen

Deposited As Hansenula polymorpha de Morais et Maia, teleomorph
Strain Designations DL-1 [NRRL Y-7560]
Application
produces 2-butanone alcohol dehydrogenase
produces formaldehyde
produces hydrogen peroxide
produces isopropanol dehydrogenase (NADP) secondary alcohol dehydrogenase
produces methyl ketones
transformation host
utilizes methanol
reduces 4-chloro-1-(4-fluorophenyl)butan-1-one
transformation host for expression of hirudin gene
Biosafety Level 1
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
Mitochondrial genome sequenced strain. RefEldarov MA, et al. Complete sequence and analysis of the mitochondrial genome of the methylotrophic yeast Hansenula polymorpha DL-1. FEMS Yeast Res. 11(6):464-472, 2011. PubMed: 21545683.
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA; Korea Research Institute of Bioscience and Biotechnology, S. Korea; Centre 'Bioengineering' RAS, Russia).
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
SSU rDNA of ATCC 26012

AAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTCCTTTACTACATGGATAACCGTGGTAATTCTAGAGCTAATACATGCTTAAAGCCCCGACTTCTGGAAGGGGTGTATTTATTAGATAAAAAATCAATGCCTTCGGGCTTTTTGATGATTCATAATAACTTTTCGAAGCTCATGGCCTTGTGCTGGAGCTGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTTCACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATATATAACGATACAGGGCCCTTTCGGGTCTTGTAATTGGAATGAGTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTGGTTAGCCGGTCTGCCTTTTTTGGCACGTACTGGATTTAACCGGGCCTTTCCTTCTGGCTAACCAACACTCTGTGTTGGCAAACCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGAATATATTAGCATGGAATAATAGAATAGGACGTTATGGTTCTATTTTGTTGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAGTTGTCAGAGGTGAAATTCTTGGATTTACTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGTGGTGTTTCTTTTTTGACCCACTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAATAGTGATATTGGCTTTTGCTGATTTTCACTTCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAACGAGCTCTATCCTTGACCGAGAGGTCCGGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGCAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTAGTACCGATTGAATGGCTTAGTGAGGCTTCAGGATTGGTTCAGAGAAGGGGGCAACTCCATCTTGGAACCGAGAATCTAGTCAAACTTGGTCATTTAGAGGAACTAAAA


ITS of ATCC 26012

TTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTCTAAGCTGGAGCTGTTAATTGATTGAACTGTTTGCCTGCGCTGCTGAAATACTGCGTGCGCGGTGATGGTTTTTTTGGTTGCAGTTCTACTCAAGGCTTAGAAATCTCCTTACACACTTTGTTTTCTTATTAGAGACAAATTTGCTTTGGCTAGCGGTCGTTTAAACGCGGCATGCCTGGCCAGAGGTTTTAACAAAACTCATTTTCGTCTTGCCCTCGTGGGAAGATTGAAAACTTGTATTATTATTTCGTCAGTTATAAGAAGTTGCGTTTGCAGCTTTTCTAAACTTTAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGATTTTCGTGAATCATTGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCCCTCTCAAACCCCCGGGTTTGGTGATGGGCAATACTCTCGTCTGAGAGTTTGCTTGAAATGAATTGGCATGAGTTTTTGATACGAACTTCAGCTGTTTATTCAATGTATTAGGTTTTATCCATCGTTGATTGACAACTGCAAGCTTCTTTCAAAATTTGGCTCTGCCTTACAAACTTTAATCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAAT


D1/D2 region of LSU rDNA of ATCC 26012

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAGCGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGAAGAAAGTAATCTTGGAGGTGGCCTTTGTCTATGTTCCTTGGAACAGGACGTCATGGAGGGTGAGAATCCCGTGTGATGAGGTGTCCATCTCCGTGTAAGATGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTTGATCAGACTTGGTATTTAGCTATCATCGCTCCTTGTGGGTGGTGCTCTAGCTTTTTACTGGGCCAGCATCAGTTTTGGTGGCAAGATAATGACAGTTGAATGTGGCTCCTCGGAGTGTTATAGCTTCTGTTGATATTGCCTACCGGGACTGAGGTCTGCGGCTTTTGCCTAGGATGCTGGCGTAATGATCCAATACCGCCCGT

Name of Depositor DW Levine
Chain of Custody
ATCC <-- DW Levine <-- CL Cooney
Isolation
Soil, Massachusetts
Cross References

Nucleotide (GenBank) : JN863704 RPB 2 gene

Nucleotide (GenBank) : JN863698 RPB 1 gene

Nucleotide (GenBank) : FJ914939 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : FJ914922 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : FJ914907 18S rRNA gene

References

Patel RN, et al. Method for producing microbial cells and use thereof to produce oxidation products. US Patent 4,266,034 dated May 5 1981

Patel RN, et al. Microbial reduction of 1-(4-fluorophenyl)-4-[4-(5-fluoro-2-pyrimidinyl)-1-piperazinyl]butan-1-one. Biotechnol. Appl. Biochem. 17: 139-153, 1993. PubMed: 8166884

Baratti J, et al. Preparation and properties of immobilized methanol oxidase. Biotechnol. Bioeng. 20: 333-348, 1978.

Kim CH, et al. Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha. Biotechnol. Lett. 18: 417-422, 1996.

Cooney CL, Levine DW. Microbial utilization of methanol. Adv. Appl. Microbiol. 15: 337-365, 1972. PubMed: 4593294

Kang HA, et al. Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene. Yeast 14: 1233-1240, 1998. PubMed: 9791894

Kushnirov VV. Rapid and reliable protein extraction from yeast. Yeast 16: 857-860, 2000. PubMed: 10861908

Bravo M, Wang DI. Enzymatic oxidation of methanol to produce formaldehyde and hydrogen peroxide. Adv. Biotechnol. 3: 329-334, 1981.

Patel RN, et al. Oxidation of secondary alcohols to methyl ketones by yeasts. Appl. Environ. Microbiol. 38: 219-223, 1979. PubMed: 42348

Patel RN, et al. Microbial production of methyl ketones. Purification and properties of a secondary alcohol dehydrogenase from yeast. Eur. J. Biochem. 101: 401-406, 1979. PubMed: 230031

Suh SO, Zhou JJ. Methylotrophic yeasts near Ogataea (Hansenula) polymorpha: a proposal of Ogataea angusta comb. nov. and Candida parapolymorpha sp.nov. FEMS Yeast Res. 10: 631-638, 2010. PubMed: 20491937

Eldarov MA, et al. Complete sequence and analysis of the mitochondrial genome of the methylotrophic yeast Hansenula polymorpha DL-1. FEMS Yeast Res. 11(6):464-472, 2011. PubMed: 21545683.

Ravin NV, et al. Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1. BMC Genomics 14: 837, 2013. PubMed: 24279325