Scheffersomyces shehatae (Buckley et van Uden) Urbina et Blackwell (ATCC® 22984)

Strain Designations: M 175 [NRC 2883]  /  Product Format: freeze-dried

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Deposited As Candida shehatae Buckley et van Uden, anamorph
Strain Designations M 175 [NRC 2883]
Application
Bioethanol production
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain

Yes

Comments Ferments xylose
Increase in ploidy during stress
Genome sequencing strain (Nanjing Forestry University, China)
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTTAGAAATAAGTTGGGCCAGAGGTTAACCAAACTTCAATTTTATTATTGAATTGTTATTTTATTTAATTTGTCAATTTGTTGATTAAATTCAAAAAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAGCCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCAGACTAGGCGTTTGCTTGAAAAGTATCGGCATGAGTAGTACTAGATAGTGCTTTCAGGATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGAATTCTTGGTAGTGAATTTTTAGTATCATGGCTCTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCAACTCTATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTCTGCAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACTACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCCTAAACCGC

Name of Depositor SA Meyer
Cross References

Nucleotide (GenBank) : JQ023135 RPB 1 gene

Nucleotide (GenBank) : HQ652024 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : HQ652065 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : JQ023147 RPB 2 gene

Nucleotide (GenBank) : KC479677 18S rRNA gene

Nucleotide (GenBank) : KC507458 EF-1 alpha gene

Nucleotide (GenBank) : KC479711 XYL1 gene

References

Alexander MA, et al. Levels of pentose phosphate pathway enzymes from Candida shehatae grown in continuous culture. Appl. Microbiol. Biotechnol. 29: 282-288, 1988.

Hinfray CT, et al. Influence of the oxygenation level on D-xylose fermentation by free and agar-entrapped cultures of C. shehatae. Appl Microbiol Biotechnol 42: 682-687, 1995

Jeffries TW. Effects of culture conditions on the fermentation of xylose to ethanol by Candida shehatae. Biotechnol. Bioeng. Symp. 15: 149-166, 1985.

Lee H, Fisher BG. Unusual fructose utilization by Pichia stipitis and its potential application. J. Ferment. Bioeng. 69: 79-82, 1990.

Palnitkar S, Lachke A. Effect of nitrogen sources on oxidoreductive enzymes and ethanol production during D-xylose fermentation by Candida shehatae. Can. J. Microbiol. 38: 258-260, 1992. PubMed: 1393828

Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624

Talbot NJ, Wayman M. Increase in ploidy in yeasts as a response to stressing media. Appl Microbiol Biotechnol 32: 167-169, 1989

Yang VW, Jeffries TW. Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae. Appl Biochem Biotechnol 26: 197-206, 1990

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation