DAKIKI (ATCC® TIB-206™)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast; Epstein-Barr virus (EBV) transforme

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Organism Homo sapiens, human
Cell Type lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Disease Carcinoma
Clinical Data
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV.
Genes Expressed
immunoglobulin; monoclonal antibody
Comments
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgA; lambda light chain
Name of Depositor P Ralph
References

Steinitz M, Klein G. EBV-transformation of surface IgA-positive human lymphocytes. J. Immunol. 125: 194-196, 1980. PubMed: 6247394

Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913

Cross References

Nucleotide (GenBank) : U07986 Human immunoglobulin IgH heavy chain Fd fragment mRNA, partial cds.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Steinitz M, Klein G. EBV-transformation of surface IgA-positive human lymphocytes. J. Immunol. 125: 194-196, 1980. PubMed: 6247394

Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913