CESS (ATCC® TIB-190)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: blood  /  Disease: myelomonocytic leukemia

Organism Homo sapiens, human
Tissue blood
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 [Cells Contain HERPESVIRUS]
Disease myelomonocytic leukemia
Age adult
Gender Male
Ethnicity Caucasian
Applications
CESS cells can be used to assay T-cell replacing factor (TRF).
Storage Conditions liquid nitrogen vapor phase
Images TIB-190 Micrograph
Derivation
Isolated from the peripheral blood of an adult Caucasian male patient with myelomonocytic leukemia after incubation with Epstein-Barr Virus.
Clinical Data male
Caucasian
adult
Receptor Expression
complement
Genes Expressed
immunoglobulin; surface immunoglobulin (sIg+); Epstein-Barr virus (EBV)
Cellular Products
immunoglobulin; surface immunoglobulin (sIg+); Epstein-Barr virus (EBV)
Comments
The cells are EBNA +.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
D5S818: 11,12
D13S317: 12
D7S820: 10,12
D16S539: 12
vWA: 16,17
THO1: 7,9.3
Amelogenin: X,Y
TPOX: 8,9
CSF1PO: 10,11

Isotype IgG
Name of Depositor P Ralph
References

Muraguchi A, et al. T cell-replacing factor-(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J. Immunol. 127: 412-416, 1981. PubMed: 6972960

Miki Y, et al. The requirement for esterase activation in T cell replacing factor (TRF)-induced IgG production in a human B blastoid cell line. J. Immunol. 128: 675-678, 1982. PubMed: 6798119

Kishimoto T, et al. IgG induction in a human B cell line by red cell-mediated microinjection of the cytoplasm from T cell factor-stimulated B cells. J. Immunol. 129: 1367-1371, 1982. PubMed: 6980930

Miki Y, et al. Induction of IgG production in a human monoclonal B lymphoblastoid cell line by a B cell-specific monoclonal antibody. J. Immunol. 129: 1921-1925, 1982. PubMed: 6981668

Bradley TR, et al. Cell lines derived from a human myelomonocytic leukaemia. Br. J. Haematol. 51: 595-604, 1982. PubMed: 6980663

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Basic Documentation
Other Documentation
References

Muraguchi A, et al. T cell-replacing factor-(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J. Immunol. 127: 412-416, 1981. PubMed: 6972960

Miki Y, et al. The requirement for esterase activation in T cell replacing factor (TRF)-induced IgG production in a human B blastoid cell line. J. Immunol. 128: 675-678, 1982. PubMed: 6798119

Kishimoto T, et al. IgG induction in a human B cell line by red cell-mediated microinjection of the cytoplasm from T cell factor-stimulated B cells. J. Immunol. 129: 1367-1371, 1982. PubMed: 6980930

Miki Y, et al. Induction of IgG production in a human monoclonal B lymphoblastoid cell line by a B cell-specific monoclonal antibody. J. Immunol. 129: 1921-1925, 1982. PubMed: 6981668

Bradley TR, et al. Cell lines derived from a human myelomonocytic leukaemia. Br. J. Haematol. 51: 595-604, 1982. PubMed: 6980663

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm