EB2 [EB-2] (ATCC® HTB-61™)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain Eptein-Barr Virus
Disease Burkitt's lymphoma
Age 7 years juvenile
Gender female
Ethnicity Black
Applications
Electron microscopic studies reveal viral particles (Epstein-Barr virus), lipid bodies, vacuoles, poorly developed mitochondria, frequent microvilli and an unusual layered structure projecting from the nucleus (a finding also reported for EB1 cells).
The established EB2 line is similar, in general, to EB1 but differs in that it is composed of larger and less uniform cells which grow more rapidly than EB1. Furthermore, the EB2 cells regularly form clumps, have more cytoplasm, a less rounded nucleus and microvilli.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypertriploid human cell line with the modal chromosome number of 80, occurring in 30% of cells and polyploidy at 4.1%. Most cells had a chromosome number ranging between 79 and 85. Seven marker chromosomes were common to all cells, including der(14)t(8;14) (q22;q24) (two copies), i(5p), der(6)t(3;6) (p21.1;p21.1), der(11)t(7;11) (q11.23;q23.1) and two others. Four other marker chromosomes occurred only once among 11 metaphases analyzed. Most cells had four normal X chromosomes. No Y chromosomes were found in QM preparation. Some cells had five copies of N1 and/or N3.
Derivation
The established EB2 line is similar, in general, to EB1 but differs in that it is composed of larger and less uniform cells which grow more rapidly than EB1. Furthermore, the EB2 cells regularly form clumps, have more cytoplasm, a less rounded nucleus and microvilli. Electron microscopic studies reveal viral particles (Epstein-Barr virus), lipid bodies, vacuoles, poorly developed mitochondria, frequent microvilli and an unusual layered structure projecting from the nucleus (a finding also reported for EB1 cells). The line is considered more primitive in differentiation than EB1.
Clinical Data
female
Black
7 years juvenile
Comments
The established EB2 line is similar, in general, to EB1 but differs in that it is composed of larger and less uniform cells which grow more rapidly than EB1. Furthermore, the EB2 cells regularly form clumps, have more cytoplasm, a less rounded nucleus and microvilli. Electron microscopic studies reveal viral particles (Epstein-Barr virus), lipid bodies, vacuoles, poorly developed mitochondria, frequent microvilli and an unusual layered structure projecting from the nucleus (a finding also reported for EB1 cells). The line is considered more primitive in differentiation than EB1.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10(5) cells/ml and maintain at between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,13
D16S539: 8,10
D5S818: 8,12
D7S820: 10,12
THO1: 9,9.3
TPOX: 8,9
vWA: 14,16
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Name of Depositor G Trempe, LJ Old
Year of Origin 1963
References

. . Br. J. Cancer 19: 108-115, 1965.

Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014

. . J. Exp. Med. 121: 761-770, 1965.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

. . Br. J. Cancer 19: 108-115, 1965.

Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014

. . J. Exp. Med. 121: 761-770, 1965.