RD-ES (ATCC® HTB-166)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: Ewing's sarcoma

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Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology epithelial
Culture Properties mixed: adherent and clusters in suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Ewing's sarcoma
Age 19 years
Gender male
Ethnicity Caucasian
Derivation
The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.
Clinical Data
19 years
Caucasian
male
Antigen Expression

Blood type B; Rh+

Comments
Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.
The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1-2
PGM3, 1
Name of Depositor G Marshall, M Kirchen
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation