SC (ATCC® CRL-9855™) Organism: Homo sapiens, human / Tissue: peripheral blood / Cell Type: monocyte/macrophage General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue peripheral blood Cell Type monocyte/macrophage Product Format frozen Morphology monocyte/macrophage Culture Properties suspension Biosafety Level 1 Storage Conditions liquid nitrogen vapor phase Derivation The SC cell line was established from human peripheral blood mononuclear cells. Comments The cells grow as single cells in suspension, and have a granular appearance. Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10% Subculturing Cultures can be maintained by addition or replacement of fresh medium. Interval: Maintain the cell concentration between 2 X 105 and 1 X 106 cells/mL Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C Name of Depositor E.I. du Pont de Nemours & Co. References Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995