H9/HTLV-IIIB (ATCC® CRL-8543™)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain RETROVIRUS
Applications
The cells produce HTLV III.
Genes Expressed
Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)
Comments
The cells produce HTLV III.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
References

Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569