143B PML BK TK (ATCC® CRL-8304)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: osteosarcoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue bone
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease osteosarcoma
Age 13 year old
Gender female
Ethnicity Caucasian
Clinical Data
13 year old
Genes Expressed
genome, partial [tk]
Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Name of Depositor Wistar Institute
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation