143B PML BK TK (ATCC® CRL-8304)

Organism: Homo sapiens, human  /  Disease: osteosarcoma

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma
Age 13 year old
Gender female
Ethnicity Caucasian
Clinical Data
female
Caucasian
13 year old
Genes Expressed
genome, partial [tk]
Comments
Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Name of Depositor Wistar Institute