C5/MJ (ATCC® CRL-8293)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: cord blood  /  Disease: Leukemia

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Organism Homo sapiens, human
Tissue cord blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Retrovirus]
Disease Leukemia
Age newborn
Gender male
Applications The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens.
Storage Conditions liquid nitrogen vapor phase
Derivation This line was established by Popovic et al. in 1983 from neonatal cord blood by co-cultivation with an HTLV-I producing cell line, MJ (see ATCC CRL-8294).
Clinical Data
male
neonatal
Comments

The cells express a cell surface glycoprotein of 61000 daltons (45000 daltons when not glycosylated) that is characteristic of HTLV infection.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at about 2 to 4 x 10cells/mL and maintain between 4 x 105 and 1 x 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 12
D16S539: 8,11
D5S818: 13
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 16,17
Name of Depositor President and Fellows of Harvard College
Year of Origin 1983
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.