Reh (ATCC® CRL-8286)

Organism: Homo sapiens, human  /  Disease: acute lymphocytic leukemia (non-T; non-B)

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Organism Homo sapiens, human
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphocytic leukemia (non-T; non-B)
Storage Conditions Liquid nitrogen vapor phase
Antigen Expression
CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
Genes Expressed
CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
D5S818: 11, 13
D13S317: 11, 13
D7S820: 9, 12
D16S539: 9
vWA: 14, 15
THO1: 7
TPOX: 8
CSF1PO: 10, 11, 12
Amelogenin: X
Name of Depositor Univ. Pennsylvania
References

Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. PubMed: 365240

Cross References

Nucleotide (GenBank) : Z35498 H.sapiens mRNA for cellular adhesion regulator.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. PubMed: 365240