AHH-1 (ATCC® CRL-8146)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  / 

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Age 33 years
Gender male
Ethnicity Caucasian
Applications
The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products.
They are are sensitive to killing by benzo[a]pyrene.
Storage Conditions liquid nitrogen vapor phase
Karyotype near diploid
Clinical Data
male
Caucasian
33 years
Comments
The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: The cells grow as floating aggregates of large cell clusters.
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Population Doubling Time 16 to 19 hrs
Name of Depositor Massachusetts Institute of Technology
References

Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985