BDCM (ATCC® CRL-2740)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: peripheral blood  /  Disease: acute myelogenous leukemia (AML)

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain EBV viral DNA sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute myelogenous leukemia (AML)
Age unknown
Gender male
Ethnicity unknown
Applications
Cells may be useful in modeling immune reactions.
Storage Conditions liquid nitrogen vapor phase
Derivation
BDCM is a B cell line established from a pheresis sample from a patient with M5a leukemia.
Clinical Data
male
unknown
Antigen Expression
CD20; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD40; Homo sapiens, expressed
CD80; Homo sapiens, expressed
CD83; Homo sapiens, expressed
CD86; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
CD1a; Homo sapiens
CD11c; Homo sapiens
Receptor Expression
interleukin 3 (IL-3)
Comments

In addition to B cell markers, the cells have many characteristics of dendritic cells.

The cells are positive for Epstein-barr virus (EBV).

They produce fascin, interleukin 1 alpha (IL-1 alpha), interleukin 2 (IL-2), interleukin 6 (IL-6), interleukin 16 (IL-16), transforming growth factor beta1 (TGF beta1), and tumor necrosis factor (TNF).

 

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 cells/mL and maintain between 3 X 105 and 3 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 10,11
D5S818: 12,13
D7S820: 9,11
THO1: 6,9.3
TPOX: 9,10
vWA: 16,17
Name of Depositor RA Steinman
References

Kharbanda S, et al. BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells. Br. J. Haematol. 119: 819-825, 2002. PubMed: 12437665

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kharbanda S, et al. BDCM: a novel B-cell line with genetic and functional similarity to dendritic cells. Br. J. Haematol. 119: 819-825, 2002. PubMed: 12437665