C13589 (ATCC® CRL-2704)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme  / 

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Organism Homo sapiens, human
Cell Type B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain EBV viral DNA sequences
Age 39 years adult
Gender female
Ethnicity Caucasian
Applications
This cell line can be used a standard for sizing.
Storage Conditions -135°C
Clinical Data
female
Caucasian
39 years adult
Comments
This cell line can be used a standard for sizing.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: -135°C
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,10
D5S818: 12,13
D7S820: 9
THO1: 6,8
TPOX: 10,11
vWA: 15
Name of Depositor SL Nolin
References

The line was established from mononuclear cells taken from a fragile-X premutation female with 31 and 59 CGG-triplet repeats in the fragile-X gene, FMR1. This allele size is the smallest undergoing expansion to a full mutation (greater than 200 repeats) in one transmission. This expansion to a full mutation with greater than 200 repeats occurs only on transmission through females. Fragile X is a common cause of inherited mental retardation and is caused by an expansion of a CGG repeat within the gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

The line was established from mononuclear cells taken from a fragile-X premutation female with 31 and 59 CGG-triplet repeats in the fragile-X gene, FMR1. This allele size is the smallest undergoing expansion to a full mutation (greater than 200 repeats) in one transmission. This expansion to a full mutation with greater than 200 repeats occurs only on transmission through females. Fragile X is a common cause of inherited mental retardation and is caused by an expansion of a CGG repeat within the gene.