J.gamma1.WT (ATCC® CRL-2679)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Disease: acute T cell leukemia

Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties clusters in suspension
Biosafety Level 2  [Cells contain CMV and SV-40 viral DNA sequences]
Disease acute T cell leukemia
Gender male
Applications
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Derivation
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Clinical Data
male
Comments
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Maintain cell density between 3 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor RT Abraham
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067

Basic Documentation
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067