J.gamma1.WT (ATCC® CRL-2679)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Disease: acute T cell leukemia

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Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties clusters in suspension
Biosafety Level 2  [Cells contain CMV and SV-40 viral DNA sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute T cell leukemia
Gender male
Applications
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Derivation
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Clinical Data
male
Comments
The J.gamma1.WT cell line was derived from the phospholipase C-gamma1 (PLC-gamma1) deficient J.gamma1 cell line (ATCC CRL-2678) by stable transfection with a wild-type expression vector, pcaPLC-gamma1.
The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene.
The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Maintain cell density between 3 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor RT Abraham
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067