DG-75 [D.G.-75] (ATCC® CRL-2625™)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma
Age 10 years
Gender male
Ethnicity White
Applications
The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
Storage Conditions liquid nitrogen vapor phase
Karyotype t(8:14)(q24;q32) RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
Clinical Data
male
White
10 years
Antigen Expression
CD10; Homo sapiens, expressed
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
CD19; Homo sapiens, expressed (weakly expressed)
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273(weakly expressed)
CD20; Homo sapiens
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
CD23; Homo sapiens
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
CD38; Homo sapiens, expressed
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
CD39; Homo sapiens
RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
Receptor Expression
Fc, not expressed
RefBen-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
complement (C3), not expressed
RefBen-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
Genes Expressed
surface immunoglobulin (IgM, k),CD10; Homo sapiens, expressed ,CD19; Homo sapiens, expressed (weakly expressed),CD20; Homo sapiens,CD23; Homo sapiens,CD38; Homo sapiens, expressed ,CD39; Homo sapiens
Comments
The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time 20 to 24 hrs
Name of Depositor H Ben-Bassat
Year of Origin 1975
References

Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

The DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitt's lymphoma.

weakly expressed

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

The DG-75 cell line was established in 1975 from a pleural effusion of a patient with a primary abdominal lymphoma, which clinically and histologically resembled Burkitt's lymphoma.

weakly expressed

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273