I 9.2 (ATCC® CRL-2571™)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte

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Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute T cell leukemia
Gender male
Applications
Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.
The I 9.2 cell line and the I 2.1 cell lines are completely resistant to Fas-induced apoptosis.
Complementation of I 9.2 cell line with wild-type caspase-8 restored Fas-mediated apoptosis.
The I 9.2 cell line is severely deficient in cell death induced by TNF-alpha and is partially deficient in cell death induced by ultraviolet irradiation, adriamycin and etoposide.
This cell line can be used to study the role of caspase-8 in apoptotic signaling pathways in the absence of overexpression.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Comments
The I 9.2 cell line is a caspase-8 mutant of the wild-type Jurkat cell line A3 (see ATCC CRL-2570).
Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.
ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks.
Two of these ICR-191 treated clones have been deposited at the ATCC. They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD.
The I 9.2 cell line is functionally defective for caspase-8 and the I 2.1 cell line is functionally defective for FADD.
The I 9.2 cell line and the I 2.1 cell lines are completely resistant to Fas-induced apoptosis.
Complementation of I 9.2 cell line with wild-type caspase-8 restored Fas-mediated apoptosis.
The I 9.2 cell line is severely deficient in cell death induced by TNF-alpha and is partially deficient in cell death induced by ultraviolet irradiation, adriamycin and etoposide.
This cell line can be used to study the role of caspase-8 in apoptotic signaling pathways in the absence of overexpression.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 2 X 10(5) and 2 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,11
D16S539: 11
D5S818: 9
D7S820: 8,9.2
THO1: 6,9.3
TPOX: 8,10
vWA: 18
Name of Depositor J Blenis
References

Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801

Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904

Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182

Ding HF, et al. Essential role for caspase-8 in transcription-independent apoptosis triggered by p53. J. Biol. Chem. 275: 38905-38911, 2000. PubMed: 10988287

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801

Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904

Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182

Ding HF, et al. Essential role for caspase-8 in transcription-independent apoptosis triggered by p53. J. Biol. Chem. 275: 38905-38911, 2000. PubMed: 10988287