BE(2)-M17 (ATCC® CRL-2267)

Organism: Homo sapiens, human  /  Cell Type: neuroblast  /  Tissue: brain; derived from metastatic site: bone marrow  /  Disease: neuroblastoma

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Organism Homo sapiens, human
Tissue brain; derived from metastatic site: bone marrow
Cell Type neuroblast
Product Format frozen
Morphology neuroblast
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 2 years
Gender male
Applications This cell line is a suitable transfection host.
Derivation
BE(2)-M17 is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a 2 year old male with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Clinical Data
Patient had repeated courses of chemotherapy and radiotherapy.
male
Comments
BE(2)-M17 cells have a reported saturation density of greater than 1 x 106 cells/cm2
The cells grow as multilayering, neuroblastic cells with occasionally long neuritic processes.
As cultures age, cells will aggregate and float.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium carefully. Add 1-2 mL trypsin (0.25%) - EDTA (0.53mM) solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, triturate cells and dispense into new culture flasks.


Subcultivation ratio: 1:20 to 1:50 at 1 to 2 week intervals is recommended
Medium Renewal: Every 4 to 7 days. pH should be less than or equal to 7.3.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 18
Population Doubling Time 20 to 24 hrs
Name of Depositor JL Biedler
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org