CA-HPV-10 (ATCC® CRL-2220™)

Organism: Homo sapiens, human  /  Tissue: prostate  /  Cell Type: human papillomavirus 18 (HPV-18) transfected

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Organism Homo sapiens, human
Tissue
prostate
Cell Type human papillomavirus 18 (HPV-18) transfected
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain human papilloma viral DNA sequences
Disease adenocarcinoma
Age 63 years
Gender male
Ethnicity Caucasian
Applications
CA-HPV-10 was derived from cells from a prostatic adenocarcinoma of Gleason Grade 4/4. The cells were transformed by transfection with HPV18 DNA. Incorporation of HPV18 DNA was confirmed by polymerase chain reaction.
Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.
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Storage Conditions liquid nitrogen vapor phase
Karyotype aneuploid; mean chromosome number at passage 26 was 72; 10% of the cells retain the double minutes seen in the source tumor
Derivation
CA-HPV-10 was derived from cells from a prostatic adenocarcinoma of Gleason Grade 4/4. The cells were transformed by transfection with HPV18 DNA. Incorporation of HPV18 DNA was confirmed by polymerase chain reaction. Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted. Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.
Clinical Data
63 years
Caucasian
male
Antigen Expression
kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens
Genes Expressed
kallikrein 3, KLK3 (prostate specific antigen, PSA)(not expressed)
Tumorigenic No
Comments
Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.
Complete Growth Medium The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    • Subculturing
    Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution; let the culture set incubate at 37C for two minutes. Neutralize the trypsin with 0.1% soybean trypsin inhibitor, and gently dislodge the cells by agitating or tapping the flask. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Name of Depositor DM Peehl
    References

    Weijerman PC, et al. Lipofection-mediated immortalization of human prostatic epithelial cells of normal and malignant origin using human papillomavirus type 18 DNA. Cancer Res. 54: 5579-5583, 1994. PubMed: 7923200

    Permits Notice: Necessary Permits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Product Sheet
    Product Sheet
    Certificate of Analysis
    Certificate of Analysis
    MSDS
    MSDS
    References

    Weijerman PC, et al. Lipofection-mediated immortalization of human prostatic epithelial cells of normal and malignant origin using human papillomavirus type 18 DNA. Cancer Res. 54: 5579-5583, 1994. PubMed: 7923200