C2BBe1 [clone of Caco-2] (ATCC® CRL-2102)

Organism: Homo sapiens, human  /  Cell Type: Enterocyte  /  Tissue: colon  /  Disease: colorectal adenocarcinoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue colon
Cell Type Enterocyte
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease colorectal adenocarcinoma
Age 72 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (ATCC® HTB-37™) by limiting dilution.
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
Clinical Data
male
72 years
Caucasian
Receptor Expression
epidermal growth factor (EGF)
Comments
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.

C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.

Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.

The cells express substantial levels of BB myosin I similar to that of the human enterocyte.

Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.



Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subculture at 85% of confluence (about every 5 to 7 days).
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11, 13, 14
D16S539: 12, 13
D5S818: 12, 13
D7S820: 11, 12
THO1: 6
TPOX: 9, 11
vWA: 16, 18
Name of Depositor MD Peterson, M Mooseker
References

Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287

Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Basson MD, et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287

Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435