DMS 153 (ATCC® CRL-2064™)

Organism: Homo sapiens, human  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Product Format frozen
Culture Properties monolayer of adherent clusters
Biosafety Level 1
Disease carcinoma; small cell lung cancer
Age 44 years
Gender male
Ethnicity Caucasian
Applications
The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.
Derivation
The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.
Clinical Data
The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.
The patient had received prior therapy with cytoxan and methotrexate.
male
Caucasian
44 years
Antigen Expression
Leu 7 +; My23 +; CD11b +
Receptor Expression
bombesin; epidermal growth factor (EGF); complement (CR3)
Genes Expressed
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol,Leu 7 +; My23 +; CD11b +
Tumorigenic Yes
Comments
The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung.
The patient had received prior therapy with cytoxan and methotrexate.
The cells express HLA class I and class II antigens.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Twice per week
Remove the spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Set up flasks at about 9 X 10 exp6 cells per 75 sq. cm. flask.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OS Pettengill, G Sorenson
References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Restrictions Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
References

Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631