T2 (174 x CEM.T2) (ATCC® CRL-1992)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr virus viral DNA sequences]
Applications

This cell line is a suitable transfection host.

Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.

Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).
Antigen Expression
HLA A2; CD7 +
Comments
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative.
The cells synthesize, but do not express, HLA B5.

T2 appears to have lost both copies of the CEMR.3 derived chromosome 6.

The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The cells synthesize, but do not express, HLA B5. 


Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 viable cells/mL and maintain between 3 X 105 and 1 X 105 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539: 10, 12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 7, 9.3
TPOX: 8, 12
vWA: 14, 17, 20
Viral Testing Tested positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR.
Name of Depositor P Cresswell
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841

Basic Documentation
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841