T1 (174 x CEM.T1) (ATCC® CRL-1991)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast,T lymphoblast  /  Tissue: lymphoblast  / 

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Organism Homo sapiens, human
Tissue
lymphoblast
Cell Type B lymphoblast,T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr virus viral DNA sequences]
Applications
T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line).
Antigen Expression
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Genes Expressed
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 +
Comments
The cells express large amounts of HLA DR7 and HLA A2.
It does not express DR3.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539:10,12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 7, 9.3
TPOX: 8, 12
Name of Depositor P Cresswell
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841