T1 (174 x CEM.T1) (ATCC® CRL-1991™) Organism: Homo sapiens, human / Tissue: lymphoblast / Cell Type: B lymphoblast,T lymphoblast General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue lymphoblast Cell Type B lymphoblast,T lymphoblast Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 Applications T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens. Storage Conditions liquid nitrogen vapor phase Derivation This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line). Antigen Expression HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + Genes Expressed HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + Comments The cells express large amounts of HLA DR7 and HLA A2. It does not express DR3. Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10% Subculturing Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Cryopreservation Freeze medium: complete growth medium, 95%; DMSO, 5% Culture Conditions Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO2), 5% STR Profile Amelogenin: XCSF1PO: 11, 12D13S317: 11, 12D16S539:10,12D5S818: 12, 14D7S820: 10, 11, 15THO1: 7, 9.3TPOX: 8, 12 Name of Depositor P Cresswell References Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841