COLO 829BL (ATCC® CRL-1980™)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 CELLS CONTAIN HERPESVIRUS
Age 45 years
Gender male
Ethnicity Caucasian
Applications
The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore.
A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Derivation
A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Clinical Data
The specimen was taken from a patient prior to chemotherapy for metastatic melanoma.
male
Caucasian
45 years
Comments
The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore.
The specimen was taken from a patient prior to chemotherapy for metastatic melanoma.
A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-4 X 10 (5) cells/ml and maintain between 2 X 10 (5) and 2 X 10 (6) cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor GE Moore
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS