H1HeLa (ATCC® CRL-1958)

Organism: Homo sapiens, human  /  Tissue: cervix  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue cervix
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain human papilloma virus (HPV-18)]
Disease adenocarcinoma
Age 31 years
Gender female
Ethnicity Black
Applications
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
Storage Conditions liquid nitrogen vapor phase
Derivation
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
Clinical Data
31 years
Black
female
HeLa Markers Y
Genes Expressed
keratin
Comments
The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).
The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Culture Conditions
Atmosphere: Air, 100%
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor RR Rueckert
References

Lee WM Ph.D. thesis, Univ. Wisconsin, 1991

Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lee WM Ph.D. thesis, Univ. Wisconsin, 1991

Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717