BUD-8 (ATCC® CRL-1554)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 56 years
Gender female
Ethnicity Caucasian
Applications
This line is highly sensitive to interferon and can be used in assays of interferon activity.
Clinical Data
female
Caucasian
56 years
Comments
This line is highly sensitive to interferon and can be used in assays of interferon activity.
The line is capable of at least 8 population doublings after thawing.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,10
THO1: 9
TPOX: 11
vWA: 16,17
Isoenzymes
G6PD, B
Name of Depositor BW Uhlendorf
References

Interferon bioassay methods and the development of standard procedures: a critique and analysis of current observations. Rockville, MD: Tissue Culture Association; 1974.

Grossberg SE, et al. Interferon standards: a memorandum. J. Biol. Stand. 7: 383-395, 1979. PubMed: 536379

Basic Documentation
References

Interferon bioassay methods and the development of standard procedures: a critique and analysis of current observations. Rockville, MD: Tissue Culture Association; 1974.

Grossberg SE, et al. Interferon standards: a memorandum. J. Biol. Stand. 7: 383-395, 1979. PubMed: 536379