166-ME SK (ATCC® CRL-1533)

Organism: Homo sapiens, human  /  Disease: hereditary adenomatosis (Gardner's variant)

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease hereditary adenomatosis (Gardner's variant)
Age 20 years
Gender male
Ethnicity Black
Clinical Data
The tissue donor suffered from Gardner's variant form of hereditary adenomatosis.
male
Black
20 years
Comments
The tissue donor suffered from Gardner's variant form of hereditary adenomatosis.
The line is capable of 16 population doublings after thawing.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
Name of Depositor L Kopelovich