EB (ATCC® CRL-1365)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender male
Clinical Data
The tissue donor suffered from a variant type of Ehlers-Danlos syndrome.
male
Comments
The tissue donor suffered from a variant type of Ehlers-Danlos syndrome.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 8 to 10 days.